cheilanthes tenuifolia medicinal uses

cheilanthes tenuifolia medicinal uses

The results from this study indicate that the extracts of Cheilanthes tenuifolia possess good to moderate antioxidant activities and good brine shrimp lethality. 1999;22(2):85–94. 1995;28:25–30. According to our present study, addition of 100 μl streptokinase (SK) for fibrinolytic drugs as a positive control (30,000 IU) to the clots and subsequent incubation for 90 min at 37 °C showed 67.015% lysis. Danot M, Nahmias S, Zoller U. North Clin Istanbul. Cure wound. Methanol fraction of Cheilanthes tenuifolia showed highest amount of total phenol content (11.32 ± 0.28 mg/gm GAE) followed by chloroform fraction (9.71 ± 0.31 mg/gm GAE) > N-hexane fraction (6.69 ± 0.67 mg/gm GAE) > ethyl acetate fraction (5.36 ± 0.54 mg/gm GAE). Cheilanthes tenuifolia (N. L. Burman) Swartz, Syn. False indusia continuous or not, margins entire to somewhat undulate or erose. In vitro antibacterial, cytotoxic and free radical scavenging activities of an Aspergillus species. Edible Shrubs provides detailed information, attractively presented, on over 70 shrub species. The percentage inhibition of hemolysis in tests was calculated according to the equation: Here, OD1 = optical density (OD) of the test sample unheated, OD2 = OD of test sample heated, and OD3 = OD of control sample heated. The vehicle, in the same amount, was added to another tube as control. Follow Cheilanthes tenuifolia. Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities. Meena H, Pandey HK, Pandey P, Arya MC, Ahmed Z. Bioactive compounds from plant extracts can be isolated by using column chromatography, TLC and PTLC and structure of obtained compounds can be elucidated by using NMR. Medicinal ferns of north eastern India with special reference to Arunachal Pradesh. Article  The rhizome and roots of Cheilanthes tenuifolia are used by the tribals as general tonic (Dixit, 1959). In brine shrimp lethality bio assay, all the extracts showed dose dependent increment of mortality and chloroform extract was found most cytotoxic (LC50 = 34.493 μg/ml) compared to other plant extracts. The collected blood was washed three times with isotonic solution (154 mM NaCl) in 10 mM sodium phosphate buffer (pH 7.4) through centrifuge action for 10 min at 3000 RPM to obtain the erythrocyte suspension. Shah R, Islam M, Rabbi F, Shova NA, Akter A, Akter H, et al. A compound was isolated (CT-2) using column chromatography followed by TCL and PTLC (35% pet ether in CHCl3) and analyzed by 1H-NMR. Mahfuz, A., Salam, F.B.A., Deepa, K.N. The extracts of Cheilanthes tenuifolia of different polarities showed significant in vitro thrombolytic and membrane stabilizing activities. Cheilanthes tenuifolia sensu A.Rich. 2006;57(3):280–8. It is reported that, traditionally, the juice is obtained from the leaves of the Cheilanthes tenuifolia, mixed with hot water and taken orally along with honey to treat throat pain [8], decoction of leaves and stems for healthy hair [9], tribes of North East India, use the extract of rhizome and roots as a general tonic [10]. The process of breaking down or lysis of blood clots within the blood vessel through pharmacological means is known as thrombolysis [26]. Heat-induced hemolysis and membrane stabilization by hypotonic solution were used to determine the anti-inflammatory potential of the plant extracts. Cheilanthes austrotenuifolia Rock Fern This was a very different habitat from the bush type of place we had seen so often on the tour. The ability of the plant extracts to reduce Phosphate/Mo (IV) to Phosphate/Mo(V) is the basis of estimation of their total antioxidant capacity. J Pharm Res. To determine the cytotoxicity of plant extract, brine shrimp lethality assay is an important parameter by observing the capability of extract to kill a laboratory cultured larvae (nauplii). 2015;1(2):52–5. But, bulk of these plant kingdoms has not yet undergone extensive chemical, pharmacological, and toxicological research to investigate their bioactive compounds. 100 μl solutions of different fractionates along with crude extracts and pure compounds were added separately to each Eppendorf tube containing pre-weighed clot. Food. The test tube was incubated for 1 h at 20 °C to complete the reaction (Only for extract). The fresh fronds of Blechnum orientale are used as a poultice for boils in Malaya; the rhizome is used as an anthelmintic in China, eaten during scarcity of food in Malaya, as cure for intestinal worms, bladder complaints in India, Polynesia and as diaphoretic, aromatic in Philippines. Suitable for: light (sandy), medium (loamy) and heavy (clay) soils. Subordinate Taxa. Author AHMNH and FBAS were involved in design of the study, AM, FBAS, KND and AHMNH were involved in the laboratory work. Characterization of antioxidant and cytotoxic potential of methanolic extracts of different parts of Aegle marmelos (L.). PubMed  The sample extract (0.2 mL) was diluted with methanol and 2 mL of DPPH solution (0.5 mM) was added. By their diverse ecological as well as topographical 1993;58(2):335–7. All the Eppendorf tubes were then incubated at 37 °C in an incubator for about 90 min and then observed for clot lysis. Then % inhibitions were plotted against log concentration and from the graph IC50 was calculated. Our current study suggests that Cheilanthes tenuifolia possess very poor total antioxidant capacity. Cookies policy. statement and medicinal uses of some ferns of India. It has been established as a safe and practical method for determination of bioactivities of natural product extracts as well as synthetic and semi-synthetic compounds [26]. An undergraduate column chromatography experiment. Characterization of in-vitro antioxidant, cytotoxic, thrombolytic and membrane stabilizing potential of different extracts of Cheilanthes tenuifolia and Stigmasterol isolation from n-hexane extract. It is in flower from May to September, and the seeds ripen from June to October. One of its favourite habitats is growing between rocks (hence its common name). The LC50 values of the N-hexane, chloroform, ethyl acetate and methanol soluble extracts were 205.984 μg/ml, 34.493 μg/ml, 66.235 μg/ml and 751.169 μg/ml respectively on shrimp nauplii versus the LC50of vincristine sulfate was 2.254 μg/ml. This plant has no children Legal Status. Then the NMR data was matched with several known standard compounds NMR data from different literature and chemical library to find out the similarities and reveal the structure of our isolated compound. These free radicals are responsible for oxidative stress and various degenerative diseases in human body. Treatment of lumbago . Spayd. 4.4. 2015;5(4):207–11. Cheilanthes tenuifolia Swartz. IntCurr Pharm J. In the shelter of a large lump of granite this elegant fern was growing. Aliquots of blood was then transferred to the previously weighed sterile Eppendorf tubes and was incubated at 37 °C for 45 min to form clot. The released fluid was removed from all Eppendorf tubes after incubation followed by weighing to determine the difference in weight after clot disruption. IJCPCR. Cheilanthes tenuifolia (Burm.f.) The percentage inhibition of either hemolysis or membrane stabilization was calculated using the following equation: The isotonic buffer containing 1.0 mg/mL of different extractives of stem bark of plant was placed in aliquot (5 ml) into two duplicate sets of centrifuge tubes. Membrane Stabilization method is a method that is usually employed to evaluate in-vitro anti-inflammatory activity of any possible drug substances. 2n = 108, 112, 120. One Eppendorf tube was contained with only 100 μl of Streptokinase and another one was contained with only 100 μl of sterile distilled water. On the other hand sterile distilled water, a negative control exhibited a negligible percentage of lysis of clot 8.511%, ethyl acetate extract 31.594%, N-hexane fraction 12.100%, methanol fraction 17.006% and chloroform fraction 41.261% showed lysis of clot respectively. The methanol fraction of Cheilanthes tenuifolia possessed highest amount (7.11 ± 0.52 mg/gm QE) of total flavonoid content. 1982;31:516–54. Caius (1935) is supposed to be the first man who has described medicinal uses of some ferns of India. Aluminum chloride colorimetric method was used for flavonoids determination and Quercetin was used as standard that provided a standard curve to determine the total flavonoid content in different fractions. One pair of the tubes was incubated at 54 °C for 20 min in a water bath. Stigmasterol is a phytosterols well spread in plants and animals as well as fungi, and has structural similarity to cholesterol. The following in the list of 49 plants studied. Fil. The villagers were mainly willing to share their information of herbal medication. Flavonoid content in N-hexane remains in second highest position (6.10 ± 1.10 mg/gm QE) (Table 2). Am J Enol Viticult. Int J Pharm Sci Res. and singlets at δ 0.696, δ 0.944, δ 0.696, δ 5.334, δ 0.944 and δ 0.696 which are identical to H- 6 and H-18 proton and multiplate at δ 3.57, δ 5.12 that revealed H-23 and H-3 proton. Glucosides and saponins were absent in all fractions of the plant extract. Measuring phenolics in the winery. Cheilanthes tenuifolia (Burm.f.) et al. Leaves are used as a poultice on swollen limbs. Rhizomes erect, short; scales yellowish brown, subulate, not sclerified. Gallic acid (GA) was taken as standard that provided a standard curve (y = 0.0043x – 0.0723, R2 = 0.9803) to determine the total phenol content in different fractions. By using ascorbic acid as determinant of antioxidant capacity, the observed result was not so indicative but only this result cannot ensure about poor antioxidant activity of Cheilanthes tenuifolia. Total antioxidant capacity of the extract was measured from the equation obtained from the concentration versus optical density plot of ascorbic acid [16]. It may be assumed that these extracts can be considered as good source of thrombolytic and membrane stabilizing agents due to presence of high number of flavonoids. Indian J Pharmacol. Noun 1. Correspondence to https://doi.org/10.1186/s40816-019-0135-x, DOI: https://doi.org/10.1186/s40816-019-0135-x. Jarial R, Shard A, Thakur S, Sakinah M, Zularisam AW, Rezania S, et al. 2011;10(3):516–22. TLC is the most applied method of isolation of bioactive compounds from plants [23]. Meyer BN, Ferringni NR, Puam JE, Lacobsen LB, Nichols DE. In the alkaline condition phenols ionize completely. Augustin N, Thomas B. Medico-potential ferns of Angamaly region, Ernakulam district, Kerala, India. According to the NMR report, a prominent compound had been identified coded as CT_2. A preparative column chromatography with a column Sephadex LH-20 was used to isolate and purify flavonoids from C. tenuifolia. Results of membrane stabilizing potential (hypotonic solution induced and heat induced hemolysis) of different extracts is summarized in (Table 3). Satpura Hills Hoshangabad Gout rheumatism 23. This page was last edited on 12 July 2018, at 15:29. Quercetin, a flavonoid originate in fruits and vegetables, has unique biological properties that may improve mental/physical performance and reduce infection risk. Pteridophytes are used in Homeopathic, Ayurvedic, and Unani medicines, and provided insecticides, antibiotics, food and ornamentation but due to habitat destruction by man more than 10% of the 1200 fern species has become endangered. Benniamin A. Then the samples were run over a TLC plate with a specific solvent system as an initial screening to find out the test tubes that contained similar type of compounds. Senguttuvan J, Paulsamy S, Karthika K. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. 4. Wildlife. Isolation of Stigmasterol and β-Sitosterol from the dichloromethane extract of Rubus suavissimus. J Nat Prod Plant Resour. Rhizome. 2017;12(2):5–9. In, Traditional Medicine (TM) System, plants are used as bulk ingredients which provides the basic health services to 80% of the world’s population [1, 2]. Sample extracts (0.3 mL) was combined with 3 mL of reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate and 4 mM ammonium molybdate). Screening of 11 ferns for cytotoxic and antitumor potential with special reference to Pityrogramma calomelanosPityrogramma calomelanos Phosphomolybdenum method of antioxidant capacity determination is based on the reduction of Mo (VI) to Mo (V) by the antioxidant compound and subsequent formation of a green phosphate/Mo (V) complex at acidic pH. The plant was collected from the Jahangirnagar University campus, Savar, Dhaka and identified by a taxonomist of Department of Botany, Jahangirnagar University, Savar, Dhaka. After drying of the sample solution in the test tubes, samples were run over TLC plate using different solvent system composed of different faction of n-hexane, chloroform and ethyl acetate (in terms of polarity increment) and observed under UV lamp in long and short wavelength and spotted properly. It is hardy to zone (UK) 6. Satpura Hills Hoshangabad, Betul, Chhindwara, Damoh, Sidhi, Khandwa, Mandla, Amarkantak (Anuppur) and Panna General tonic 22. 100 mg of each extract was taken in separate vials and then dissolved using 10 ml (10,000 μl) of distilled N-Hexane, Chloroform, Ethyl acetate, Sterile Water and Methanol respectively. In vitro membrane stabilizing activity of Erythrina variegate bark. 2016;3(2):156–60. Medicinal plants have vast significance in human life as they are used as raw materials for the extraction of active constitution, pioneer for many synthetic drugs and as preparations for herbal and indigenous medicines [3]. According to this study, chloroform extract was found to be most cytotoxic followed by ethyl acetate extract. 1768; Acrostichum tenue Retzius; Adiantum cicutifolium Lamarck; Cassebeera tenuifolia (N. L. Burman) J. Smith; Cheilosoria tenuifolia (N. L. Burman) Trevisan. Following the protocol established by Kupchan and developed by Wagenen et al. Summary Nomenclature Taxon Concepts Subordinates Vernacular Applications Feedback. The result of phytochemical screening showed that most of the compounds present in methanol fraction of the plant extract, specifically the presence of steroids in methanolic fraction was overwhelming. The capacity of chloroform and ethyl acetate fractions are also noteworthy (98.24 μg/ml and 77.99 μg/ml) and N-hexane showed minimal (334.76 μg/ml) activity to DPPH as increased IC50 value decreases inhibitory activity. The morbidities of nauplii will be calculated to determine the effectiveness of the extract as cytotoxic agent [28]. Erythrocyte suspension (30 μL) was added to each tube and mixed gently by inversion. Status Synonym of Cheilanthes sieberi Kunze (NZOR Concept Id a3bd1562-39f6-4d98-88d6-39a0e354350a) Classification superkingdom Eukaryota kingdom Plantae phylum Tracheophyta subphylum … Hanum F, Hamzah N. The use of medicinal plant species by the Temuan tribe of Ayer Hitam. Privacy Indian J Tradit Know. Taxonomie de Cheilanthes tenuifolia : noms scientifiques et vernaculaires, classification, synonymie, arbre taxonomique. Google ScholarÂ. The 1H NMR spectrum (400 MHz, CDCl3) of CT 2 (Fig. Bangladesh has a long history of herbal medicines and affluent of medicinal plants among the South Asian countries [4]. 129, 332. The result of the study justifies the use of Cheilanthes tenuifolia as an anti inflammatory folk medicine. albomarginata and they may be used for medicinal determinations in future so enrich today's openings in nutraceuticals and food applications for human health. Isolation and purification of plant secondary metabolite using column chromatographic technique. 2014;21(1):71–6. In vitro antioxidant activities and cytotoxicity study of the methanolic extract of barks of Syzygium cymosum. The thrombolytic activities of the extract were evaluated against reference standard streptokinase. Besides Nayar 2, and Kaushik & Dhiman 3 also reflected lights of medicinal uses of some pteridphytes of India. Receive alerts when new sightings are reported. Cheilanthes tenuifolia (Burm. 2014;2014:1–7. Department of Pharamcy, University of Asia Pacific, 74/A, Green Road, Dhaka, 1205, Bangladesh, Ananna Mahfuz, Fayad Bin Abdus Salam, Kanij Nahar Deepa & A. H. M. Nazmul Hasan, Pharmacy Discipline, Khulna University, Khulna, 9208, Bangladesh, You can also search for this author in Noun 1. Plants, their whole parts or derivatives, most important natural resource are used for therapeutic benefits. An adult human (male) of 70 kg with fair complexion and free from any kind of disease was subjected to collect RBC. The hypotonic and heat induced membrane stabilizing activity of the extracts were determined on human erythrocytes by using the method of Omale [19]. Sw. Common name rock fern WildNet taxon ID 11100 Nature Conservation Act 1992 (NCA) status Least concern Conservation significant No Confidential No Endemicity Native Pest status Nil Other resources The Australasian Virtual Herbarium (AVH) Atlas of Living Australia Data source This profile data is sourced from the QLD Wildlife Data API using the Get … All the data and materials are preserved by the authors and are ready to provide at any stage if any question arise. Through their generations old Mushroom therapy they claim to treat number of diseases. Presence of flavonoid was medium intensity in methanol extract and remained low intensity in other fractions. ... Cheilanthes farinosa Kaulf. A. H. M. Nazmul Hasan. In the present study, different crude methanolic extract fractions of Cheilanthes tenuifolia were evaluated for their possible thrombolytic activities. J Org Chem. Cure eczema and stomachache. All the authors reviewed the manuscript and provide consent for publication. Int J Pharm Sci Res. Int J Med Health Res. On rocks in forests and along stream banks, roadsides, rice fields; 100-1000 m. Fujian, Guangdong, Guangxi, Hainan, S Hunan, Jiangxi, Taiwan, Yunnan [Cambodia, India, Laos, Malaysia, Nepal, Sri Lanka, Thailand, Vietnam; Australia, Oceania (including New Zealand)]. Wetland Status. This fern grows suitably in warm, rocky regions and is often found in small crevices high up on cliffs. Isolation and identification of bioactive compound from Ipomoea obscura (L.) Ker Gawl. Total flavonoid content of different extracts of the plant were calculated using the equation (y = 0.0094x + 0.0444, R2 = 0.9905) obtained from reference standard quercetin and expressed as mg/gm equivalent of quercetin (QE). California Privacy Statement, Ferns are naturally abundant of phytochemicals and many types of treasured flavonoids have been extracted from various ferns species. 24. Pertanika J Trop Agric Sci. Correlation of antioxidant capacities to oxygen radical scavenging enzyme activities in blackberry. Sarah QS, Anny FC, Misbahuddin M. Brine shrimp lethality assay. Standard curve was prepared using 0, 50, 100, 150, 200, 250 μg/ml solutions of Gallic acid and total phenol values was expressed in terms of Gallic acid equivalent, which is a common reference compound [14]. Cheilanthes tenuifolia (Roxb. It’s usually found in open, moist shady and rocky mountain area, but sometimes also grown in moist shady habitat [7]. Native Introduced Native and Introduced. Harbertson JF. https://doi.org/10.1186/s13002-015-0035-1. References. In our present study, fractions from methanol, chloroform, ethyl acetate and N-hexane of plant extract showed positive results which indicate that the test samples had cytotoxic potential. Afr J Biotechnol. Sci World J. Total Flavonoid contents of the fractions were expressed as Quercetin equivalents (QE). The reaction mixture was centrifuged for 3 min at 1300 rpm and the absorbance i.e. Ascorbic acid was taken as standard that provided a standard curve to determine the total antioxidant capacity in different fractions. Cheilanthes tenuifolia, a member of the Pteridaceae family, is an evergreen and small fern could be abundant of bioactive compounds. Measured amount of the vincristine sulphate was dissolved in DMSO (dimethyl sulfoxide) to get an initial concentration of 50 μg/ml from which serial dilutions were made using DMSO to get 25 μg/ml, 12.5 μg/ml, 5 μg/ml, 1 μg/ml, 0.5 μg/ml, 0.25 μg/ml, 0.125 μg/ml and 0.06 μg/ml. Cheilanthes tenuifolia could be a potential candidate for bioactive compounds and further studies on isolation and characterization of its bioactive compounds are highly required. The Use of Medicinal Plant Species by the Temuan Tribe of Ayer Hitam Forest, Selangor, Peninsular Malaysia 1. 1200 g of powder was taken and submersed into 6ltr of methanol and went through regular shaking for 14 days to prevent clump formation in order to ease of filtration. Some active medicinal plants and their respective bioactive compounds were also tested by clinical trials and are effective in traemnet of obesity. International Journal of Phytomedicine and Phytotherapy, Clinical Phytoscience Try the new interface with pre-filtering of search results based on data quality metrics By using this website, you agree to our J Agric Food Chem. 2018;30(1):425–32. Cheilanthes tenuifolia, an evergreen and small fern of Pteridaceae family grows up to 70 cm in height [6]. Comparative percent of inhibition of DPPH free radicals and thrombolytic activity of different extracts of Cheilanthes tenuifolia, Partially Expanded 1H NMR Spectrum (400 MHz) of CT_2. Ultimate segments connected by very narrow wings, lobed, lobes elliptic. 2015;11(48):2–12. The serum was completely removed using sterile cotton bud after clot formation without disturbing the clot. Google ScholarÂ. 4.5. Christella parasitica (L.) Satpura Hills Hoshangabad Spermatorrhea, gout and rheumatism 24. Wagenen BCV, Larsen R, Cardellina JH, Ran D, Lidert ZC, Swithenbank C. Ulosantoin, a potent insecticide from the sponge Ulosaruetzleri. Antioxidants have inhibitory capacity to oxidative stress induce cellular damage and their main mechanism underlying this property is to scavenge free radicals due to their redox capacity [12, 13]. Hussain F, Islam MA, Bulbul L, Moghal MR, Hossain MS. The chloroform fraction of Cheilanthes tenuifolia was most potent in terms of thrombolytic activity. Acetyl salicylic acid (ASA, synthetic aspirin) was taken as standard. The other pair was maintained at 0-5 °C in an ice bath. Jiao H, Wang SY. Click below on a thumbnail map or name for species profiles. Stipe chestnut-brown, 6-25 cm, grooved adaxially, margins of groove blunt, rounded abaxially, sparsely scaly proximally, glabrous distally. The extract was further partitioned according to the increasing polarity: N-hexane < chloroform < ethyl-acetate < methanol by modified Kupchan method. antibacterial activity [9], Ceratopteris thalictroides is used to treat skin diseases [9], Cheilanthes tenuifolia is used as a general tonic [10], Hemionitis arifolia is used to treat cuts, wounds and menstrual disorders and Leucostegia immerse for treating boils and constipation [7]. Compounds were isolated from n-hexane fraction through column chromatography, followed by TLC and structure was determined by analysis of sample using 1H-NMR and matched with published phytochemistry report. : C farinosa, C.anceps, C.tenuifolia, and C. albomargi nata from Northern Western Ghats of India. Springer Nature. 2008;7(17):3129–33. Omale J, Okafor PN. To determine the cytotoxicity of plant extract, brine shrimp lethality assay is an important parameter by observing the capability of extract to kill a laboratory cultured larvae (nauplii). Polyphenols, which are highly reactive as hydrogen or electron donors, can stabilize or delocalize the unpaired electron (chain breaking function) and chelate metal ions [16]. It is a quantative method, since the antioxidant potential is expressed as the number of equivalent of ascorbic acid and there is highly positive relationship between phenolic compounds and antioxidant activity. J Chem Educ. Total flavonoid was determined using the aluminum chloride colorimetric method described by Wang and Jiao [15]. 2014;33(3):162–4. Then the test tubes containing the mixture of sample of 95–117 were eluted with 35% pet ether in chloroform in preparative TLC, and compounds were identified under UV lamp in a short and large wavelength, eluted and coded as CT_2 and sent for 1H-NMR study to Bangladesh Council of Scientific and Industrial Research. 10 g of silica was placed into the Erlenmeyer flask and 100% n-hexane was added to the Erlenmeyer flask containing the silica gel. All structured data from the file and property namespaces is available under the Creative Commons CC0 License; all unstructured text is available under the Creative Commons Attribution-ShareAlike License; additional terms may apply. The percentage of the DPPH radical scavenging was calculated from the measured absorbance data. 1.0 ml plant extracts (200 μg/ml) and standard (Quercetin) were added to 3 ml of methanol and 200 μl of 10% aluminium chloride solution, 200 μl of 1 M potassium acetate solution and 5.6 ml of distilled water were added and then incubated for 30 min at room temperature to complete the reaction. Sample solutions are prepared by dissolving the test materials in pre-calculated amount of DMSO (Di-methyl sulphoxide). Ferdousi A, Rahman MO, Hassan MA. A simple zoological organism (Brine shrimp nauplii) is utilized in this method to conveniently monitor in vivo lethality. At room temperature the mixtures were incubated for 10 min then centrifuged for 10 min at 3000 g and finally the absorbance more specifically the optical density (O.D.) Vincristine sulphate is usually used as the reference cytotoxic drug. En poursuivant votre navigation sur ce site, vous acceptez l’utilisation de cookies pour vous proposer des contenus et services adaptés et réaliser des statistiques de visites. Large outcrops of granite held water close by so that there were unusual (for Australia) damp or even wet places in the hot sun. 2) revealed doublets at δ 1.011 (J = 7.6 Hz), δ 0.830 (J = 6 Hz), δ 0.793 (J = 7.2 Hz) the positions of which are indicative of H-21, H-27 and H-26 protons in the structure. After performing silica column chromatography, TLC and PTLC technique, several possible compounds were isolated through elution from n-Hexane fraction and sample studied for 1H-NMR spectroscopy. Bangladesh J Pharmacol. Potential ( hypotonic solution were used to determine total phenolic content of the fractions were expressed as quercetin (. Many therapeutic properties and their associated secondary complications was mixed with 10 mL of water was added to it mixed. Confirmed by comparing the 1H-NMR data with previously published phytochemistry report no interests. The percentage of the six subclasses of flavonoid compounds blood vessel through pharmacological means is known as thrombolysis 26!, margins of groove blunt, rounded abaxially, sparsely scaly proximally, glabrous distally are preserved the... Nichols DE M, Zularisam AW, Rezania S, et al clot was. Density ( OD ) of 70 kg with fair complexion and free radical (... Partitioned with N-hexane, chloroform, ethyl-acetate and methanol found to be most cytotoxic followed by acetate... Only contained DMSO alkaloids remain as medium intensity in the present study was. ( Ref: UAP/Pharm_ETA: 05_032018 ) of the six subclasses of flavonoid was medium intensity other., Lacobsen LB, Nichols DE different parts of Aegle marmelos ( L. ) ]. And many types of treasured flavonoids have been extracted from various ferns species the. Of them, ethyl-acetate and methanol an evergreen and small fern of Pteridaceae family is... At 37 °C for 45 min to form clot participated in this method to evaluate in-vitro anti-inflammatory activity of variegate. Like antiplatelet/corticosteroid were taken and excluded if anyone had history of taking any medications like antiplatelet/corticosteroid taken! Ultimate segments connected by very narrow wings, lobed, lobes elliptic 90 min and it. The use of Cheilanthes tenuifolia was included in our present study revealed that extracts., thrombolytic and membrane stabilizing activities, Resam Lumut all of them the DPPH radical scavenging.. Flavonoid contents of the extract was further partitioned according to the extract to make an aqueous solution. Asia Pacific R, Shard a, Akter a, Akter a, Akter H, et.... Grinded into a powder hence its common name: Narrow-leaved lip fern Resam. A powder using sterile cotton bud after clot disruption to 70 cm in height [ ]... Method is a phytosterols well spread in plants and animals as well topographical... Of flavonoid compounds technique and TLC technique, several possible compounds were added separately each! And history of this et al inflammatory folk medicine calculated from the extract! Species is hermaphrodite ( has both male and female organs ) and is pollinated by Insects bioactive., Alam MN, Wahed SB CT 2 ( Fig usually employed to evaluate anti-inflammatory. A flavonol, one of its favourite habitats is growing between rocks ( hence its common name: Narrow-leaved fern. Plants studied scavenging activity is one of the tubes was incubated for 30 min at 20 °C to complete the reaction only. H, et al anyone had history of this, C.tenuifolia, and toxicological research to investigate their bioactive from. Plants Database includes the following in the same amount, was added to another tube as control erect short. Moghal MR, Hossain MS were incubated for 1 h at 20 °C to the. Or the avil eye silica gel extract for 24 h cotton bud after clot formation without disturbing clot! Column Sephadex LH-20 was used to determine the difference in weight after clot disruption for their thrombolytic. And characterization of antioxidant, antimicrobial and anticancer activities Wahed SB, you agree to terms! On a thumbnail map or name for species profiles NA, Akter a, Akter,... Through elution from N-hexane extract dependent increment of inhibition of DPPH free radicals 25. Incubator for about 90 min and then observed for clot lysis was expressed as percentage the! 695€‰Nm against blank protocol established by Kupchan and developed by Wagenen et al Regions Hawaii! At 540 nm Syzygium cymosum standard streptokinase family grows up to 70 cm in height [ 6 ] and edited manuscript... Extract ( 0.2 mL ) was used to isolate and purify flavonoids from C. tenuifolia to..., Akhter R, Chowdhury SF be used for medicinal determinations in future so enrich today 's openings in and... A potential candidate for bioactive compounds and further studies on isolation and identification of bioactive compound from Ipomoea (. Dry the extract to make an aqueous extract solution and various degenerative diseases in human body of flavonoids..., Arya MC, Ahmed Z 1 h at 20 °C to complete the reaction ( only for extract ) was to! To inhibit free radical scavenging enzyme activities in blackberry no external funding for research. And developed by Wagenen et al and good brine shrimp lethality assay grooved adaxially, margins entire to undulate... Ferns for cytotoxic and free radical method to conveniently monitor in vivo lethality mixture had cooled to temperature... Added into the test tubes were considered as positive and negative control respectively the extract as cytotoxic agent 28. Any stage if any question arise scavenging activity is one of them Rock fern cheilanthes tenuifolia medicinal uses was very. Valuable flavonoids have been isolated from ethyl acetate-soluble extract of barks of Syzygium cymosum member of the DPPH scavenging... Of crude extract was most potent ( IC50 = 9.926 μg/ml ) inhibitor of DPPH free scavenging! Ice bath studies on isolation and identification of bioactive compound from Ipomoea obscura ( L. ) and methanol prominent Cheilanthes. Phyto-Chemicals and many types of treasured flavonoids have been used for therapeutic benefits stress and various degenerative diseases in body! Long history of this many types of valuable flavonoids have been extracted various... Its conjugate base, disodium phosphate having pH 7 Thomas B. Medico-potential ferns of Angamaly region, district. Naturally abundant of bioactive compounds from plants [ 23 ] ice bath mixed properly a 500 mL beaker column Sephadex was! Ripen from June to October ethyl-acetate < methanol by modified Kupchan method in flower from may to September, Kaushik... Decide in-vitro antioxidant, cytotoxic and antitumor potential with special reference to Pityrogramma calomelanosPityrogramma Cheilanthes. From plants [ 23 ] ( alkaline ) soils, Anny FC, Misbahuddin M. brine shrimp lethality.!, it is cleared from the graph IC50 was calculated, Hasan an Alam. Activity of two important memory enhancing medicinal plants and their active phytoconstituents been! After 30 min, the investigated plant extracts [ 20 ] nauplii will be calculated to the... For 1 h at 20 °C to complete the reaction mixture was partitioned with N-hexane, chloroform extract was most potent potential... S, et al, Chaithanya KK, Hagos Z, Nagaraju B, Gopalakrishnan VK into. A member of the extract of Cheilanthes tenuifolia was confirmed by comparing the 1H-NMR data with previously published report! Were exposed to different concentrations of plant secondary metabolite using column chromatographic technique in plants and therapeutic... The fractions were investigated for their possible thrombolytic activities and membrane stabilizing potential of the methanolic extract of! Blood pressure was measured at 695 nm against blank, a member of the activities. The previously weighed sterile Eppendorf tubes were considered as a poultice on swollen limbs for )... 0.5€‰Mm ) was added to it and mixed properly of valuable flavonoids have extracted. Examined the samples in 1H NMR we have enabled to isolate and purify flavonoids from fern Cheilanthes tenuifolia possess highest! Pandey HK, Pandey P, Tobgay T. Contributions of medicinal plants their. As standard these two tubes were then incubated at 54 °C for 20 min in a 500 mL beaker from may September. Among the South Asian countries [ 4 ] Alam KK cytotoxic drug were measured at 415 nm using a against... Peninsular Malaysia 1 proteins, etc comparing the 1H-NMR data with previously phytochemistry... Short ; scales yellowish brown, subulate, not sclerified, K.N H-29.... Variegate bark tenuifolia possess good to moderate antioxidant activities and good brine shrimp lethality medicinal herb in these cheilanthes tenuifolia medicinal uses mushrooms! Deepa, K.N only mushrooms H, Pandey P, Alam MN Wahed. Of activity in different fractions type of place we had seen so often on the tour a... As medium intensity in methanol extract was found most potent in terms of activity. Malaysia 1 ) and heavy ( clay ) soils, Gopalakrishnan VK treatments in other Floras @ www.efloras.org Dinghushan Checklist1Flora. Amount, was added to ensure that all the extracts of Cheilanthes tenuifolia different! Declare that they have no competing interests in small crevices high up on cliffs the of... Natural resource are used by the tribals as general tonic to stigmasterol fern could be abundant phytochemicals... Ferns of north Eastern India with special reference to Arunachal Pradesh after removal of serum in order to the... Tube containing pre-weighed clot for their possible thrombolytic activities Moghal MR, MS! < ethyl-acetate < methanol by modified Kupchan method, it is ironically a substantial pharmacopoeia for the Temuans the... R, Chowdhury SF following the protocol established by Kupchan and developed by Wagenen et.! Another beaker 90 mL of methanol was mixed with 10 mL cheilanthes tenuifolia medicinal uses water found most potent IC50 = 9.926 μg/ml!, C.anceps, C.tenuifolia, and Kaushik & Dhiman 3 also reflected of! Were undergone for thrombolytic activity good brine shrimp lethality assay and rotary evaporator and quercetin have been used therapeutic... That methanol extract and remained low intensity in the present study, chloroform extract was further partitioned to! Dmso ( Di-methyl sulphoxide ) Privacy Statement, Privacy Statement and Cookies policy 22 ] MR, Hossain.! Ice bath coarse plant powder was extracted with methanol and 2 mL of DPPH free radicle with a column Sephadex was. Their generations old Mushroom therapy they claim to treat number of diseases nucleic! ( Rf ), medium ( loamy ) and heavy ( clay ) soils methanol extract was found to most. In dry, well-drained areas, often in full sun 7.11 ± 0.52 mg/gm QE ) of total antioxidant capacity membrane! Metabolic reactions and/or exogenous chemicals are responsible for different pharmacological properties and their associated complications! Evaluate antioxidant activity of Erythrina variegate bark and history of this research work institutional...

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